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Lysates were incubated on ice for 20 minutes and cleared from insoluble debris by centrifugation (10,000 x g , 30 min, 4 C), loaded onto Ni 2+ -NTA agarose beads (Qiagen, #30210), and incubated for 1 h at 4 C

WooJMShinDYLeeSJJoeYZhengMYimJHet alCurcumin protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase-1 expression and reduction of reactive oxygen

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