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GHK Cu is a synthetic coppertripeptide complex composed of glycyl L histidyl L lysine (GHK) chelated with copper(II), used in research to study tissue remodeling, extracellular matrix regulation, and copper dependent signaling in vitro and in animal models

[61] [62] Inactivation of SOD2 in mice causes perinatal lethality

10.1016/j.tibs.2012.11.009 51 LiuD.SongZ.WangX.OuyangL

After 30 min, a 100 L of acidic stop solution was added into each well and the absorbance at 450 nm was recorded using a using a Biotek Synergy Neo2 HTS Multi-Mode Microplate Reader (Mnnedorf, Switzerland)

Invert the vial and add the correct amount of solution to the syringe