Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing IGK and IGL chains are

IGK and IGL chains are shown

DriverMap™ AIR RNA Full-length TCR-BCR Profiling Reagent Kit (Human)

a compatible  Universal Unique Dual Index (UDI) NGS Index Plate  must be purchased separately as a required component of the workflow

top-ranking genes from the Cancer Genome Atlas

and TRDs are shown

Human Genome-Wide CRISPRi Dual-sgRNA Library DriverMap Targeted RNA Sequencing IGK and IGL chains arePooled library of ~105,000 dual sgRNA constructs targeting ~19,000 human protein coding genes. Each construct expresses two sgRNA targeting the same gene. Designed to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression. Five CRISPRi dual sgRNA constructs target each gene. Designs based on Horlbeck, et al., eLife 2016; 5: e19760 DOI: 10. 7554). Manual and Product Documentation Manual: