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We noted that the lung to body weight ratio was indeed elevated in AA-exposed mice compared to PBS and melatonin-only controls, however the extent of this increase was substantially reduced in those mice which received melatonin alongside AA ( Figure 1B )

However, treatment with NaHS significantly ameliorates these abnormalities, whereas treatment with propargylglycine (a specific inhibitor of H 2 S) further aggravated such alterations (Jia et al., 2020)

The GCLC floxed mice (Cre-negative) shows lowered levels of glutathione in blood probably because introduced loxp sequences interrupt GCLC expression (Fig