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Mice were fed customized control chow diet containing 0.5% bile salt and 10% fat, or high cholesterol diet (CH diet, chow diet supplemented with 0.1% purified cholesterol) (Trophic, Nanjing, China, TP23302)

Specifically, this included substantial upregulation of TANC2, an mTOR regulator present in neurons (Kim et al , 2021b), Syntaxin1A (STX1A) that mediates the release of neurotransmitters from the synapse and the postsynaptic protein HOMER1 that interacts with RYR2 which was also upregulated (Fig 6C)