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The protein samples were digested by adding Trypsin (Thermo Scientific) at a 1:20 ratio (w/w) and incubated at 37C overnight

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Although HCoV-NL63 also uses the ACE2 entry receptor, the receptor-binding domain (RBD) of HCoV-NL63 is structurally very different from that of SARS-CoV-2, which would limit antibody cross-reactivity