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Population doubling was calculated as below: Determination of infection conditions for CRISPR-pooled screen The Brunello CRISPR knockout library contains an average of 4 guides per gene for a total of 77,441 sgRNAs and 1000 control guides

Unlike harmless commensal bacteria, microbial pathogens release an array of toxins or virulence factors that can alter cellular physiology and cause the release of DAMPs 1 , whose detection is instrumental in activating innate microbe-killing mechanisms

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CS, Chondroitin sulfate

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