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Statistical analysis All the statistical analyses of the data were performed using Microsoft Excel 2010 for Window 7 (Microsoft, Redmond, WA, USA) with the add-in software Statcel2 (OMS, Saitama, Japan)

Research areas relevant to canine joint health include: Cartilage Protection: Some studies suggest potential chondroprotective effects in animal models Modulation of matrix metalloproteinase (MMP) activity that degrades cartilage Potential effects on synovial fluid composition and joint lubrication Anti-inflammatory effects in joint tissues Pain and Inflammation: Observed reductions in pain behaviors in some animal studies Anti-inflammatory effects that may reduce joint swelling Potential neuroprotective effects relevant to pain signaling Mobility and Function: Functional outcome measures in research animals have shown: Improved weight-bearing on injured limbs Enhanced range of motion in joint injury models Faster return to normal activity patterns Common Canine Orthopedic Conditions Several orthopedic conditions in dogs might theoretically benefit from peptide research

a , Single-nucleus RNAseq (snRNAseq) of Gclc transcripts from the indicated cells present in human mammary tissue, from previously published data 72

We also gratefully acknowledge the support and use of facilities at the Morphology and Imaging Shared Facility and Regenerative Medicine Shared Facility provided at the Shriners Childrens Boston

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