Harvesting human foreskin fibroblast cells (HFF-1) HFF-1 (passage #1517) was cultured in DMEM medium supplemented with fetal bovine serum (12% v/v), penicillin (100 IU/mL) and streptomycin (100 g/mL) at 37C in a 5% (v/v) CO 2 and harvested at 95% confluence using 0.12% v/v trypsin-EDTA
5 Thus, the two-edged trait of ROS allows for the exertion of survival or death of cancer cells based on the intracellular levels of ROS
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doi: 10.18632/oncotarget.2600 87 SuMZhangZZhouLHanCHuangCNiceEC
