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Supernatant containing proteins were collected for immunoblotting, extracted proteins (2040 g) were separated by SDS-PAGE (615%) gel and then electroblotted onto Polyvinylidene Fluoride (PVDF) membranes (Amersham Hybond-P, GE-Healthcare Life Science, Pittsburgh, PA, USA)

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This study was approved by the Ethics Committee for Animal Experimentation of the University of Barcelona (CEEA-UB

At higher levels of performancewhether in sport, business, or longevitythe difference comes down to something far more technical: Signaling, timing, and restraint