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Collectively, these results suggested that the inhibition of ferroptosis promotes autophagy in hyperosmolarity-exposed HCECs

Western blotting was used to measure Trx system expression in lung cancer cell lines, while MTT assays were used to measure cell proliferation

Furthermore, insulin abolished glucocorticoid stimulation of surfactant production in co-cultures of rat lung fibroblasts and epithelial cells (296)

Immediately after sonication, the mixed micelle solution was dispensed into black 96-well microtiter plates (100 l/well) for (Corning) fluorescence measurements

While GHK has been shown to inhibit the inflammatory response and epithelial-mesenchymal transition (EMT) via the TGF-1/Smad 2/3 and IGF-1 pathways in bleomycin-induced fibrosis models [24], its broader anti-inflammatory effects in the context of pulmonary fibrosis pathogenesis may warrant further exploration