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Timepoint Month 6 What to check Full re-check of baseline labs plus body composition Why Most modern practitioner protocols re-evaluate the cycle around this point

Au et al., 2022b)

Measurement of bound sulfane sulfur A whole brain homogenates were prepared with 9 volumes of ice-cold buffer consisting of 10 mM potassium phosphate (pH 7.4), 1% TritonX-100, 10 mM hydroxylamine, which was used to suppress the activity of PLP-dependent enzymes involved in enzymatic H 2 S production, and protease inhibitor cocktail complete (Roche Diagnostics, Mannheim, Germany) using a Potter type glass homogenizer with a Teflon pestle (1,500 rpm, 10 strokes)

doi: 10.1016/j.micpath.2021.105067 373 GuXZhuJ

Figure 2 2.1.1 Effects on innate immune system Neutrophils are the most abundant leukocytes population in peripheral blood, and they exert their activity early during inflammation, releasing cytokines, proteases, producing reactive oxygen species (ROS), and creating neutrophils extracellular traps (NETs) (33) Neutrophils represent the main targets of A1AT action: being an anti-protease, its primary effect is to inhibit serine proteases deriving from degranulating neutrophils including NE, cathepsin G and PR3 (34)