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CAFS, Combined Assessment of Function and Survival

The newly bred variety ZZ9 demonstrated a superior profile, characterized by a more targeted transcriptional reprogramming with fewer differentially expressed genes (DEGs) yet significant enrichment in core pathways like response to water deficit. This efficient response suggests a pre-adapted mechanism that minimizes metabolic cost while activating critical defenses, in contrast to the broader, less optimized reactions of ROC22 and ZZ2

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The quality and relevance of these samples are crucial for downstream RNA extraction and analysis