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System calibration was performed according to the manufacturers instructions before each test

One interpretation of these results is that supplemental L-GSH restores cytokine balance by lowering ROS directly and by interrupting the previously mentioned cycle of continuous GSH depletion

The findings (Figure 6C) suggest that Lactobacillus and Akkermansia , which are considered potentially beneficial genera, exhibited a significant negative correlation with colonic pro-inflammatory cytokines (TNF-, IL-1, IL-6), MDA and serum LPS, and a significant positive correlation with colonic antioxidant indicators (GSH-Px, SOD)

In the group comparisons of each treatment, the MSstatTMT package quantified the log 2 fold change and estimated the standard error for each protein across treatment groups