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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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Pathways associated with regional emphysema severity Using linear mixed-effect models, the expression levels of 127 genes were significantly associated with Lm and thus associated with regional emphysema severity (Figure 2a

The apparent permeability coefficient for DSIP penetration of BMEC monolayers was in a range similar to water-soluble substances (e.g

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