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Isolating RNA, high-throughput sequencing, and data analysis were done by Seq Health Technology (Wuhan, China), following standard protocols

Before staining, the slices were washed in 0.1 M PBS thrice and incubated for 1 h in blocking solution (4% Donkey Serum, 0.3% Triton X-100 in 0.1 M PBS)

In addition, a Class II randomized clinical study evaluating chorea after tetrabenazine withdrawal reported that the discontinuation of tetrabenazine was significantly related to reemergent chorea [15]

The sgRNA targeting sequence and the following PAM sequence are highlighted in yellow and green

To solve these questions enhanced efforts will be required to characterize biochemically and anatomically the MCS proteomes in time and space 176