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Therefore, when cerebral ischemia occurs, NCOA4 may promote the release of iron ions and trigger ferroptosis in cells (105)

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Handelman SK

Wu B, Chen Y, Chen Y, Xie X,Liang H, Peng F,Che W

After washing with TBS buffer containing 0.1% (v/v) Tween 20 (TBST), the samples were incubated with fluorescent secondary antibody (1:100, Cat# SA00013-2, Proteintech, China) diluted in PBS containing 0.1% (v/v) Tween 20 for 1 h at room temperature, stained with DAPI, and observed under fluorescence microscope (Zeiss Apotome)