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A candidate for a potential glutathione-scaffold residue was identified using the following selection criteria: The residue should be rather conserved in enzymatically active in contrast to inactive glutaredoxins and have a charged side chain to ensure a strong ionic glutathione interaction (Fig

This is particularly relevant for critical-dose drugs such as anticoagulants, antiepileptics, and immunosuppressants

Finally, cells were washed in PBS and then mounted in fluorescence mounting medium (Fluoromount Aqueous Mounting Medium, refractive index 1.4)

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