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Animal procedures were approved by the Institutional Animal Care and Use Committee and complied with state, federal, and NIH regulations

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Nicotine can be delivered in a variety of ways, including: Combustible tobacco (cigarettes, cigars) Smokeless tobacco (chew, snuff) E-cigarettes and vapes Nicotine replacement therapy (patches, gum, lozenges) Nicotine pouches (oral dissolvable forms like Zyn) Each method has its own risk profile, but they all share one thing in common: nicotines interaction with the nervous and endocrine systems, which can affect hormones, including testosterone

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In line with the general pattern for all daily smokers, men were more likely than women to be heavy smokers, as can be seen in Figure 5