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GT was actively involved in drafting the manuscript

To probe for the potential direct association of these GEFs and GAPs with Retromer, we turned to AlphaFold2 modelling 102,103,104

At the end of fixation cells were washed with Ca 2+ /Mg 2+ PBS and treated with monoclonal rabbit antibodies against N-terminal extracellular domain of amyloid precursor protein (dilution in PBS to the concentration 3.84 g/mL, Abcam Limited, Discovery Drive, Cambridge Biomedical Campus, Cambridge, United Kingdom, ab126732) at 4 C overnight

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