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Firstly, it is based on the NHANES database, which employs a multistage complex probability sampling design and weighted adjustments, enhancing the representativeness and reliability of the study resultsa crucial factor for improving the generalizability of our findings within the American context

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Secondary antibodies: Fluorescein donkey anti-rat IgG (Jackson ImmunoResearch, Cat#712-095-150, 1:5000), Fluorescein donkey anti-mouse IgG (Jackson ImmunoResearch, Cat#715-095-150, 1:5000), Anti-mouse IgG, HRP-linked (Cell Signaling Technology, Cat#7076, 1:5000), Anti-Rabbit IgG, HRP-linked (Cell Signaling Technology, 7074, 1:5000), Normal mouse IgG (Santa Cruz Biotechnology, Cat#sc-2025, 2g), Normal rabbit IgG (Cell Signaling Technology, Cat#2729S, 2g), Fluorescein (FITC)-AffiniPure Donkey Anti-Rabbit (Jackson Immuno, cat# 711-095-152, 1:5000), Cy3 Goat Anti-Rabbit (Jackson Immuno, cat# 111-165-003, 1:5000), Cy3-AffiniPure Donkey Anti-Mouse (Jackson Immuno, cat# 715-165-150, 1:5000)