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Subjects were required to consume the entire quantity of designated water following exercise ad libitum within 30 min (T000 to T030 min)

Critically, the cytokines we will assay using the described DBS protocol have been validated against plasma obtained through a venipuncture from the same individuals collected at the same time using standard protocols from the field ( R 2 = 0.82

Proteins were analysed by western blotting using the respective antibodies presented in Supplementary Table 2 and either LLC Western Lightning Plus ECL (Revvity Health Sciences) for chemiluminescent signal or a Li-Cor Odyssey CLx for fluorescent signal

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Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were both implemented by KOBAS software (version: 2.1.1) for annotated genes, and the cutoff value of the corrected p -value at 0.05 aimed at assessing the statistical significance of enrichment