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Also, the Mediterranean diet can be used by gut flora to make essential metabolites such as SCFAs, these metabolites improve ENS function, thereby enhancing gastrointestinal motility (143)

Thus, the assay is less user-friendly when compared with spectrophotometric assays

EFSA Journal, 14(3), 4451

Extraction: We can now synthesize these molecules in a lab (creating synthetic peptides ) or extract them from porcine or bovine sources (creating natural bioregulators )