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After that, the slides were kept in lysing solution overnight at 4 C (100 mM Na 2 EDTA, 2.5 M NaCl, 10 mM Tris pH 10 with 10% DMSO and 1% Triton X-100 added fresh)

found that the transcription factor ChREBP can also promote BCKDK and inhibit PPM1K expression to inhibit BCKDH activation and promote ATP citrate lyase (ACLY) activation, upregulate the lipid synthesis pathway, and induce hepatic steatosis in the obesity model of high-sugar diet