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All libraries were sequenced on an Illumina HiSeq-2500 sequencer (Illumina, San Diego, CA, USA) using version 3 chemistry and flow cells with runs of 2 100 bases

While tobacco use among adolescents declined by 42.5 per cent between 2010 and 2019 according to the Global Youth Tobacco Survey, the absolute number of users remains staggeringly high, driven by Indias massive population base and rising youth demographic. The report paints a picture of success but glosses over the fact that, in real numbers, India still witnesses the addition of millions of new tobacco users every year

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Many called for the city to worry about enforcing the current laws, like the recent move restricting tobacco sales to people over 21, over instituting new bans