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In the 1980s PM routinely performed chemical analysis of smoke [36],[37], cytotoxicity testing (Ames and neutral red uptake tests) for mutagenicity [38],[39], and in vivo rodent toxicology studies [40][42], honing the techniques for performing these assays at their Institut fr Biologische Forschung (INBIFO) in Germany and Contract Research Center (CRC) in Belgium [13]

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