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Human and macaque tissue sections were pretreated for 30 min with HIER at 95 C in ER2 (Leica) followed by a 15-min protease treatment and then hybridized with human-specific probes to detect mRNA transcripts for CALCR (483048), PRLH (526568-C2) and GLP1R (519828- C3

It was reported that DRAM1 aggravates the intestinal epithelium damage of IBD patients and mouse models through increasing the activation of JNK/c-Jun pathway, but the expression of DRAM1 shows no significant change in the intestinal tissues with acute inflammation (Zhang et al., 2021)

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A systematic approach combining unbiased global metabo-lomics and gene microarray identified the folate-methionine cycle and its directly tethered de novo purine biosynthesis pathway as the cellular metabolic target of MOTS-c [4]

MRI provides high-resolution images of both soft tissues and bones, making it useful for identifying subtle injuries