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Lefkovits initially followed the Dutton-Mishell protocol which used 1ml macrocultures, but subsequently 'began to dilute macrocultures to obtain microcultures' (figure 22)

The secondary antibodies (listed in Additional file 3: Table S3) employed to visualize the localization of primary antibodies were Cy3-conjugated goat anti-rabbit IgG (1:1000)

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