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After washed with IP buffer for three times, and the binding complex proteins were eluted from the beads heated at 95 C for 10 min in 1 Laemmli sample buffer, and separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

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How concentration works (the basic math) The formula is simple: Concentration = Total peptide (mg) / Total water (ml) For a 10mg vial: 10mg / 1ml = 10mg/ml 10mg / 2ml = 5mg/ml 10mg / 3ml = 3.33mg/ml On a U-100 insulin syringe, 100 units equals 1ml