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It can be deeply frustrating when your dog seems to be eating reasonably well but keeps losing weight, has recurring loose stools, or never quite seems to thrive

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In this assay format, the antigen presenting cells are loaded with the test protein in the absence of T cells, washed, and then autologous T cells are added back at a later time point, ensuring that the function of the test protein has no impact on the outcome of the assay

Spindle abnormalities were minimal at early time points but increased significantly by 24 hours in untreated oocytes