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Three phenomena leading to oxidative stress in the brain, and implicated in neurodegenerative diseases, include inhibition of mitochondrial metabolism, neuronal excitotoxicity, and neuroinflammation [35]

In other studies, zinc deficiency exacerbates hepatic lipid metabolism, while Zn supplementation increases hepatocyte activity and improves lipid metabolism in the liver [77, 78]

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Absorbance is measured at 450 nm using a microplate reader

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