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( b and c ) Sample gating strategy for negative cells (b) and positive cells (sensor-expressing cells, c)

Cryo-EM - model building, refinement, and analysis After visual inspection of the final map, known crystal structures of Nedd4-2 domains, namely C2 (PDB: 2NSQ) and HECT (PDB: 2ONI), were fitted using the jiggle-fit tool in Coot 0.9.8 61

doi: 10.1016/j.alcohol.2007.11.003 82 WirleitnerBSchroecksnadelKWinklerCSchennachHFuchsD