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Both cell lines were grown in 250 mL flasks on an orbital shaker (110 rpm) in darkness at 25 C 1 C and sub-culturing at 7 days intervals

For the metabolomic extraction of spent media, 50 l of cell media were deproteinized by 1:16 dilution in a solution composed of 80% acetonitrile and 20% water

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These molecules act as signals, indicating the presence of stress in the brain

They found rats treated with CAPE had significantly less histologic alterations, lower ALT and AST levels, suggesting CAPE could protect against radiation-induced liver damage [47]