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Description

Chloroform (100 L per sample) was added, followed by centrifugation at 12,000 g for 15 min at 4 C to allow phase separation

In addition, in vitro experiments revealed that celastrol and Fer-1 protected against RSL3-induced HK-2 cell injury, maintained renal iron homeostasis, and alleviated ROS levels, lipid peroxidation, and ferroptosis