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Results CPZ-induced demyelination in ex vivo rat sagittal brain slice cultures is not enhanced with exogenous activation of inflammation To assess the effect of a 72-h treatment of CPZ with or without the exogenous addition of inflammatory stimulators, the expression of myelin proteins MBP and MOG was examined and quantified 24 h post-treatment in slices either untreated or treated with CPZ, CPZ + cytokines (TNFa + IFNg), CPZ + LPS, cytokines alone or LPS alone (Figure 1A)

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