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Analysis of unfiltered raw data (~35M read average per sample) was completed using the Simple Annotation of Metatranscriptomes by Sequence Analysis 2.0 (SAMSA2) pipeline [21] as follows: PEAR (version 0.9.10) to merge reads, Trimmomatic (version 0.36) to trim low-quality reads, ShortMeRNA (version 2.1) to remove rRNA, and DIAMOND (version 2.0.2) to annotate mRNA data to the RefSeq database [22]

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The clinical trial protocol explicitly allowed for delayed escalation based on tolerability

Its absolutely critical to remember that B12 injections are a Prescription-Only Medicine (POM) in the UK

A laboratory synthesizes it for the first time, discovers it seems to have interesting properties, and spends many years studying it in rodents