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The concentration of denaturant used for pellet washing is determined empirically and should be below the concentration required for solubilization of the recombinant protein

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doi: 10.1186/s13568-023-01602-2 114 LiuSYaoSYangHLiuSWangY

In the B16F10 model, for CD45 + cells, Fgf2 mRNA levels were significantly upregulated, and Fgfr2 mRNA levels were decreased in the VEGFR2-Fcexpressing tumors compared with in the Mock tumors, although the level changes were small (Fig