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Isotope-labeled 15 N and 15N-13C SERF2 proteins for NMR studies were produced by growing cells in M-9 minimal media supplemented with 100% 15 N NH 4 Cl (1 g/L) for the 15 N labeling or 15 N NH 4 Cl and D-Glucose-13C 6 (4 g/L) for the 15N-13C labeling