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Due to these adverse effects, the technique is no longer used in the United States and Europe

In locally advanced disease, such combinatorial approaches may theoretically enhance treatment responses in preclinical models, which could provide a conceptual basis for less invasive interventions

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Upon elution of the supermix bound fraction, both columns were neutralized and re-equilibrated by flowing 100% Buffer C (1X Seppro Neutralization Buffer, Sigma) in tandem over both columns at 2 mL/min for 18 min, followed by re-equilibration with 100% Buffer A for 20 min