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Description

Oxidative stress drives shared pathological processes in aging and tumorigenesis through multiple mechanisms 97

GPx4 shares the amino acid motif of selenocysteine, glutamine, and tryptophan (catalytic triad) with other GPxs

Reactions contained 0.8 mM substrate (arogenate or prephenate) and 0.8 mM cofactor (NADP + or NAD + ) together with reaction buffer [25 mM HEPES pH 7.6, 50 mM KCl, 10% (v/v) ethylene glycol]