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Description

Viability assay Cell viability was measured using PrestoBlue reagent (Invitrogen-Life Technologies GmbH, Darmstadt, Germany), according to manufacturer's protocol

The main potential issue with one-component systems is in the formation of dimers, where the intermolecular reaction occurs

Alternative formulations, such as iron protein succinylate, may be more tolerable than ferrous salts, but are untested in RLS patients as of May 2026 (Pantopoulos 2024)

7 Although mushrooms are the most concentrated sources of EGT, trace amounts of EGT have been reported in beans, oats, and organ meats such as kidney and liver