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Quantitative PCR was performed by using SYBR Green master mix (Takara, China) on a real-time PCR system (Biorad, USA)

Yang et al., 1997)

However, its crucial to note that a one-size-fits-all dosage doesnt exist

( C ) Plasma erythroid microvesicles determined by flow cytometry from WT and 4.2 / mice treated with either vehicle or mitapivat (100 mg/kg/d) for 6 months