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Images were taken with a Zeiss LSM710 confocal microscope and quantified using Fiji ImageJ

Recombinant human Eotaxin/CCL11 (300-21, PeproTech) and Eotaxin/CCL11 monoclonal antibody (MAB320, R&D systems) for neutralization were used in cell experiments

In summary, the GFE is a critical step that ensures treatments are performed safely, ethically, and effectively while complying with legal standards, providing the best possible outcome for our clients