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Henriques FS (2010) Changes in biomass and photosynthetic parameters of tomato plants exposed to trivalent and hexavalent chromium

wolthers klower ysa benefits link Wolters Kluwer Employee Perks & Wolters Kluwer: We are People

For PCR analysis, 2 L of the lysate was used as a template with the following primers: F1: 5-GCCAGCTACTGCATATATTGAGACC-3 R1: 5-GGATATAGAGAAGCCCTAGAACAGGG-3 R2: 5-CATGTCGTGTTGGTCAAATAAGCAC-3 PCR amplification using primers F1/R1 generated a 278 bp product for wild-type (WT) alleles and a 382 bp product for floxed alleles